Identifikasi Bakteri Bioaerosol di Laboratorium Mikrobiologi UIN Sunan Ampel Surabaya Menggunakan Analisis Gen 16S rRNA

Ninik Fadhillah; Wifaqwudd Az-Zahra Rifti; Yuanita Rachmawati; Inggrit Tyautari; Ade Syafira Fatau; Muh. Ma’arif

doi:10.29080/biotropic.v10i1.2540

Authors

Ninik Fadhillah Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia
Wifaqwudd Az-Zahra Rifti Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia
Yuanita Rachmawati Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia
Inggrit Tyautari Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia
Ade Syafira Fatau Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia
Muh. Ma’arif Fakultas Sains dan Teknologi, UIN Sunan Ampel Surabaya, Surabaya, Indonesia

DOI:

https://doi.org/10.29080/biotropic.v10i1.2540

Keywords:

Bioaerosols bacteria, microbiology laboratory, 16S rRNA

Abstract

Bioaerosols are airborne particles containing living microorganisms or microbial fragments that can disperse in indoor environments. This study aimed to identify the morphological characteristics and community composition of airborne bacteria in three laboratories at UIN Sunan Ampel Surabaya using a combination of macroscopic and microscopic observations and 16S rRNA gene-based molecular analysis. Air samples were collected using the settle plate method and analyzed for spatial distribution, colony morphology, and taxonomic identification. Results showed variation in colony density among the laboratories, with Gram-positive bacteria (Staphylococcus and Bacillus) dominating at 71.4%, while Gram-negative bacteria (Pseudomonas and Moraxella) accounted for 28.6%. Molecular analysis confirmed species identification with sequence similarity ranging from 97.63% to 99.31%. These findings indicate that indoor bacterial communities are influenced by interactions between environmental sources and human activity. Proper ventilation management, surface hygiene, and biosafety procedures are essential to maintain air quality and minimize occupational health risks in laboratory settings.

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